rabbit anti wnt3a  (Proteintech)

Journal: JBMR Plus

Article Title: Proteomic analysis and effects on osteogenic differentiation of exosomes from patients with ossification of the spinal ligament

doi: 10.1093/jbmrpl/ziaf021

Figure Lengend Snippet: (A) Western blots revealing Runx2 and Wnt3a expression at wk 2 and 3 following culture in osteogenic differentiation medium using exosome-penetrating (1.2 μm) and exosome-non-penetrating (0.03 μm) filters. (B) Expression levels of Runx2 and Wnt3a of OLF-derived and non-OLF-derived (control) cells co-cultured with 0.03- and 1.2-μm filters. ( n = 3 each). ** p <.01.

Article Snippet: The membranes were washed twice in PBS solution containing 0.05% Tween 20, then blocked by a mixture of 5% skimmed milk in PBS for 1 h at room temperature, and finally incubated overnight with one of the following antibodies at 4 °C: rabbit anti-Runx2 polyclonal Ab (dilution, 1:250, Proteintech) and rabbit anti-Wnt3a polyclonal Ab (dilution, 1:250, Proteintech) to assess the osteogenic differentiation, and rabbit anti-COLIVA1 polyclonal Ab (dilution, 1:250, GeneTex) rabbit anti-FMNL3 polyclonal Ab (dilution, 1:250, Proteintech), rabbit anti-mTOR polyclonal Ab (dilution, 1:250, Proteintech), or rabbit anti-PIP4K2B polyclonal Ab (dilution, 1:250, Proteintech) to confirm the differential expression of candidate proteins.

Techniques: Western Blot, Expressing, Derivative Assay, Control, Cell Culture

Journal: JBMR Plus

Article Title: Proteomic analysis and effects on osteogenic differentiation of exosomes from patients with ossification of the spinal ligament

doi: 10.1093/jbmrpl/ziaf021

Figure Lengend Snippet: (A) Western blots revealing Runx2 and Wnt3a expression at wk 2 and 3 following culture in osteogenic differentiation medium using exosome-penetrating (1.2 μm) and exosome-non-penetrating (0.03 μm) filters. (B) Expression levels of Runx2 and Wnt3a of OLF-derived and non-OLF-derived (control) cells co-cultured with 0.03- and 1.2-μm filters. ( n = 3 each). ** p <.01.

Article Snippet: The membranes were washed twice in PBS solution containing 0.05% Tween 20, then blocked by a mixture of 5% skimmed milk in PBS for 1 h at room temperature, and finally incubated overnight with one of the following antibodies at 4 °C: rabbit anti-Runx2 polyclonal Ab (dilution, 1:250, Proteintech) and rabbit anti-Wnt3a polyclonal Ab (dilution, 1:250, Proteintech) to assess the osteogenic differentiation, and rabbit anti-COLIVA1 polyclonal Ab (dilution, 1:250, GeneTex) rabbit anti-FMNL3 polyclonal Ab (dilution, 1:250, Proteintech), rabbit anti-mTOR polyclonal Ab (dilution, 1:250, Proteintech), or rabbit anti-PIP4K2B polyclonal Ab (dilution, 1:250, Proteintech) to confirm the differential expression of candidate proteins.

Techniques: Western Blot, Expressing, Derivative Assay, Control, Cell Culture

Journal: Cell Death Discovery

Article Title: TM7SF2-induced lipid reprogramming promotes cell proliferation and migration via CPT1A/Wnt/β-Catenin axis in cervical cancer cells

doi: 10.1038/s41420-024-01975-8

Figure Lengend Snippet: A The gene set enrichment analysis (GSEA) was exerted to explore the signaling pathways related to CPT1A. B , C Western blotting analysis of WNT/β-catenin signaling pathway-related protein expression after TM7SF2 overexpression and TM7SF2 knockdown in C33A cells. D , E Western blotting analysis of WNT/β-catenin signaling pathway-related protein expression after TM7SF2 overexpression and TM7SF2 knockdown in SiHa cells. F Western blotting analysis of WNT3A and β-catenin protein expression in TM7SF2-overexpressed C33A cells after MSAB treatment. G Western blotting analysis of the effect of CPT1A-overexpressed regulated the protein expression of WNT3A, β-catenin and c-Myc in TM7SF2-slienced C33A cells. H Western blotting analysis of WNT3A and β-catenin protein expression in TM7SF2-overexpressed SiHa cells after MSAB treatment. I Western blotting analysis of the effect of CPT1A-overexpressed regulated the protein expression of WNT3A, β-catenin in TM7SF2-slienced SiHa cells.

Article Snippet: Rabbit anti-human WNT3A (A0642) antibody was obtained from ABclonal (China).

Techniques: Protein-Protein interactions, Western Blot, Expressing, Over Expression, Knockdown